Diagnosis of genetic mutations using oligonucleotide microchips
Ivanov, I.B.; Ershov, G.M.; Barskiĭ, V.E.; Bel'govskiĭ, A.I.; Kirillov, E.V.; Kreĭndlin, E.I.; Parinov, S.V.; Mologina, N.V.; Mirzabekov, A.D.
Molekuliarnaia Biologiia 31(1): 159-167
1997
ISSN/ISBN: 0026-8984 PMID: 9173236 Document Number: 476317
Sequencing by means of hybridization with oligonucleotide microchip makes it possible to analyze a DNA sequence based on the results of its hybridization with a set of nucleotides immobilized on a microchip, a gel template fixed on glass support. This approach was used to detect beta-thalacemic mutations by means of hybridization of fluorescently-labeled DNA with a set of immobilized octa- and decamers or after staining of hybridized DNA by ethidium bromide. It was shown that the potentials of the microchip method increased when a special method of continuous hybridization was used. The results of the study suggest that sequencing by means of hybridization with oligonucleotide microchip can be widely used in medical diagnostics.