The properties of the proteins and nucleic acids of 3 Pseudomonas syringae phages
Boĭko, A.L.; Semchuk, L.I.; Tokarchuk, T.V.; Romashev, S.A.; Kolomiĭtseva, L.A.
Mikrobiolohichnyi Zhurnal 59(5): 28-33
1997
ISSN/ISBN: 0201-8462 PMID: 9480014 Document Number: 472920
The 9B, 123, 788/8 Pseudomonas syringae phages were investigated. PAAG electrophoretic profiles of phage proteins were identical for all three phages except the minor polypeptide having molecular weight 35,000 Da. The band corresponding to this protein was present only in 9B and 788/8 phage protein profiles. Amino acid composition of phage proteins varied insignificantly showing prevalence of Asp, Glu, Ala, Leu. Phage DNA fragments electrophoresis, carried out after processing with specific endonuclease Hind III, made it possible to evaluate common restriction sites in phage genomes. Genome molecular weight was equal to 15 mda for 9B phage and to 14 mDa for 123 and 788/8 phages. The analysis of phage growth cycle showed that latent period consisted of 50 min at 20 degrees C and the yield equalled to 70 virions per infected bacterium cell. The similarity of the phages' features suggests their broad spreading in the environment.