Growth inhibition of cultured human leukemia cells by 3-aminothymidine and its analogue
Asano, S.; Yokoyama, Y.; Kohda, K.
Anticancer Research 17(2a): 823-826
1997
ISSN/ISBN: 0250-7005 PMID: 9137414 Document Number: 472625
In a previous report, we demonstrated that 3-aminothymidine (1) strongly inhibits the growth of the human T-cell acute lymphoblastoid leukemia cell line CCRF-HSB-2 In order to further study cell growth inhibition by this compound, several of its derivatives and analogues were synthesized and their growth inhibition activities examined using various cultured cell lines. Compound 1 was the most active among the compounds tested and the most effective against cells of the human T-cell acute lymphoblastoid leukemia cell line CCRF-CEM. 3-Methylthymidine (2) also inhibited the growth of CCRF-CEM cells but at a level about one thirtieth that of 1. Introduction of a methyl or acetyl group at the 3-amino group of 1 resulted in the loss of growth inhibition activity. 3-Amino- and 3-methyl-5-bromo-2'-deoxyuridines (6 and 7), the analogues of 1 and 2, both exhibited an ability to inhibit cell growth and their levels of activity were similar in extent, in spite of the difference in their 3-amino and 3-methyl groups, however, these levels were less than that of 1. Compounds 1, 2, 6 and 7 all showed evidence of growth inhibition in every human leukemia cell line examined.