Direct colorimetric monoclonal antibody enzyme immunoassay for estradiol-17 beta in saliva
Tamate, K.; Charleton, M.; Gosling, J.P.; Egan, D.; Ishikawa, M.; Fottrell, P.F.; Kane, M.M.
Clinical Chemistry 43(7): 1159-1164
1997
ISSN/ISBN: 0009-9147 PMID: 9216451 Document Number: 471091
We developed a direct microtiter plate enzyme immunoassay to measure estradiol-17 beta in saliva. The assay has a commercially available monoclonal antibody, raised against estradiol-17 beta-6-carboxymethyloximebovine serum albumin, and a homologous horseradish peroxidase conjugate measured colorimetrically. The detection limit (equivalent to B0-3 SD) is 365 amol/well or 7.3 pmol/L when 50-microL samples are assayed. Cross-reactivity with estrone and estriol, testosterone, or progesterone is < 0.2%. Estradiol-17 beta was measured in daily samples over five natural menstrual cycles and eight cycles stimulated as a preliminary to in vitro fertilization, and the concentrations and fluctuations found agreed with previously published data. This method gives results in approximately 3 h and may be useful for fertility monitoring and management.