Effect of sodium glycyrrhetinate on chemical peritonitis in rats
Bao, J.F.; Wu, Y.J.; Yang, Y.J.; Li, X.F.
Zhongguo Yao Li Xue Bao 18(3): 277-280
1997
ISSN/ISBN: 0253-9756 PMID: 10072951 Document Number: 470864
AIM: To study the anti-inflammatory mechanisms of sodium glycyrrhetinate (SG). METHODS: Rat chemical peritonitis was used. The protein content and prostaglandin E-2 (PGE-2) content in exudate were measured by Folin-phenol assay and RIA, respectively. SOD activity in neutrophils (Neu) was determined by pyrogallol-NBT colorimetry. cAMP content in Neu was detected by competitive protein binding assay. RESULTS: In peritonitis caused by histamine, SG 10-20 mg cntdot kg-1 im reduced exudate volume and Neu counts, and 5-20 mg cntdot kg-1 im lowered the protein content in exudate. In peritonitis induced by carrageenan, SG 20 mg cntdot kg-1 im reduced exudate volume, Neu counts, protein content and PGE-2 content in exudate, increased SOD activity in Neu, but did not affect beta-glucuronidase release from Neu. In peritonitis induced by arachidonic acid, SG 20 mg cntdot kg-1 im reduced Neu counts, protein content, and PGE-2 content in exudate, and attenuated the reduction of cAMP level in Neu. CONCLUSION: SG exerts its anti-inflammatory action by lowering permeability of capillaries in inflammatory site, inhibiting Neu emigration and PGE-2 biosynthesis, and scavenging oxygen free radicals.