Effect of epidermal growth factor on cultured rat hepatocytes poisoned by CCl4
Wu, B.; Wang, C.W.; Xu, J.R.; Zhu, J.Q.
Zhongguo Yao Li Xue Bao 18(2): 176-179
1997
ISSN/ISBN: 0253-9756 PMID: 10072976 Document Number: 470536
AIM: To study the effects of epidermal growth factor (EGF) on CCl-4-induced primary cultured hepatocytes injury. METHODS: Alanine amino- transferase (AlaAT) and aspartate aminotransferase (AspAT) activities and K+ concentractions were determined by the Auto-biochemistry Assay System. Malondialdehyde (MDA) was determined by thiobarbituric acid method. Radioactivity was determined by liquid scintillametry. Light microscopy and electron microscopy were used. RESULTS: EGF 40 mu-g cntdot L-1 decreased CCl-4 (10 mmol cntdot L-1)-induced damages of rat primary cultured hepatocytes by decreasing AlaAT and AspAT leakage and MDA production, and promoted RNA and DNA synthesis, with a high positive correlation between intracellular K+ leakage and DNA syntheses (r = 0.99, P lt 0.01). Cytopathological study showed that EGF d damage of liver cells. CONCLUSION: EGF maintains the stability of cellular lipid membrane and promotes syntheses of RNA and DNA of hepatocytes, and intracellular K+ transferase is a promotor of the message transmission of DNA synthesis.