Keratinocytes cultured under hyperthermal conditions secrete factor (s) which can modulate dermal fibroblast proliferation and extracellular matrix production
Fujimoto, N.; Itoh, Y.; Tajima, S.; Ishibashi, A.
Acta Dermato-Venereologica 77(6): 428-431
1997
ISSN/ISBN: 0001-5555 PMID: 9394974 Document Number: 470484
We have studied how keratinocytes cultured under hyperthermal conditions modulate skin fibroblast growth potential and their biosynthetic phenotypes in vitro. When keratinocytes were cultured at 30, 34, 37 or 39degreeC, the conditioned medium of the keratinocytes cultured at 39degreeC showed a greater inhibitory activity for fibroblast proliferation and greater synthetic activities of collagen and glycosaminoglycans than those incubated at 30, 34, or 37degreeC. Transforming growth factor (TGF)beta1 production in skin fibroblasts was also stimulated by the keratinocyte conditioned medium cultured at 39degreeC. The stimulating activity TGFbeta1 production. The results indicate that keratinocytes cultured at a higher temperature (39degreeC) may secrete factor(s) which modulate both fibroblast growth and matrix synthesis. This may provide evidence that under hyperthermal conditions epidermis can influence the functions of skin fibroblasts.