Codon reading properties of tRNA variants substituted within the anticodon loop
Ouchi, R.; Takai, K.; Yokoyama, S.; Takaku, H.
Nucleic Acids Symposium Series 37: 115-116
1997
ISSN/ISBN: 0261-3166 PMID: 9586026 Document Number: 470441
Effects of base substitution within tRNA anticodon loop on the codon reading activities were quantitatively analyzed with the use of a set of unmodified tRNA molecules with GGA anticodon. The first (position 32) and the last (position 38) nucleotides of the anticodon loop of the wild-type molecule was changed from C32A38 to U32A38, U32G38, and C32G38. The codon reading activities of these variants relative to that of the wild type molecule were measured in a cell-free translation system. The reading of both the UCU and UCC codons were lower in all the three variants than in the wild-type molecule.