The N-acetylgalactosamine and lactosamine specific lectin from Iris hybrida leaves

Ferens-Sieczkowska, M.; Orczyk-Pawiłowicz, M.; Morawiecka, B.

Acta Biochimica Polonica 44(2): 301-307

1997


ISSN/ISBN: 0001-527X
PMID: 9360719
Document Number: 470149
The lectin isolated from the leaves of Iris hybrida binds specifically N-acetyl-galactosamine and lactose. Its molecule consists of two identical subunits bound by disulfide bonds. The lectin is a glycoprotein containing about 12% of sugars. It binds asialoglycoproteins containing complex type sugar chains. The binding is reduced by half at the concentration of 0.15 to 0.40 mM of the galactose containing disaccharides irrespectively to a type of galactose isomer. This indicates rather broad specificity of I. hybrida leaf lectin.

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