SLTR, a new family of retroviral vectors for gene transduction
Choulika, A.; Nicolas, J.F.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 190(1): 153-158
1996
ISSN/ISBN: 0037-9026 PMID: 8881275 Document Number: 469004
In this article, we describe two novel methods to modify the genome of mammalian cells. The first method utilizes a new family of recombinant retroviruses (RRV) which leads to transduction in the mammalian genome of, not a complete recombinant retroviruses, but a single LTR (sLTR) that carries the foreign gene. Therefore, all cis viral regulatory elements of the virus, including those necessary for the control of transcription, reverse transcription and integration, are absent from the transduced material. In addition to being safer because of their inability to retrotranspose, the sLTR family of recombinant retrovirus (sLTR-RRV) overcome the problems associated with the presence of viral sequences that negatively control the expression of the transduced material in stem cells.