A preliminary study of the relation between topoisomerase I and translocation (15;17)
Dong, S.; Tong, J.; Chen, S.
Zhonghua Zhong Liu Za Zhi 18(2): 88-92
1996
ISSN/ISBN: 0253-3766 PMID: 9206036 Document Number: 468449
The chromosomal translocation (15;17) which produced the PML-RAR-alpha fusion gene had been found in acute promyelocytic leukemia (APL) cells. It was identified that the RAR-alpha gene on chromosome 17 and PML gene on chromosome 15 were involved in this translocation. For study the molecular mechanisms of t(15;17) in APL, we cloned and sequenced the junctional region of the chromosomal reciprocal translocation in one APL patient. Furthermore, we compared 21 junctional sequences which had been reported with the consensus sequence of the DNA-topoisomerase II-binding sites. Thus, we proposed a hypothesis that topoisomerase II may play certain role in t(15;17) illegitimate recombination.