Induction of bimodal programmed cell death in malignant cells by the derivative Ukrain (NSC-631570)
Liepins, A.; Nowicky, J.W.; Bustamante, J.O.; Lam, E.
Drugs under Experimental and Clinical Research 22(3-5): 73-79
1996
ISSN/ISBN: 0378-6501 PMID: 8899308 Document Number: 468315
Selective induction of malignant cell death is one of the major goals of effective and safe chemotherapy. Recent developments in the understanding of programmed cell death (PCD) or apoptosis are expected to provide new leads for a safer chemotherapy. The authors investigated whether the semisynthetic alkaloid thiophosphoric acid derivative Ukrain (NSC-631570) could induce PCD or apoptosis in human K562 leukaemia cells. Results showed that Ukrain induced two distinct modalities of cell death programmes. One modality corresponded morphologically to classical apoptosis or PCD characterized by blabbing and shedding of membrane vesicles with concomitant 51Cr release; however, the Ukrain-induced apoptosis was not associated with the characteristic nuclear DNA fragmentation. Higher concentrations of Ukrain induced a second cell death programme characterized by cell surface blister formation, high specific 51Cr release and extensive DNA polyploidy. These two cell death programmes are distinct from each other in that they are interphased by a silent period characterized by normal cell morphology and reduced specific 51Cr release.