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Detection of homologous DNA sequences in animal adenoviruses by polymerase chain reaction

Kiss, I.; Matiz, K.; Allard, A.; Wadell, G.; Benkö, M.

Acta Veterinaria Hungarica 44(2): 243-251

1996

ISSN/ISBN: 0236-6290
PMID: 8908748
Document Number: 467512
A primer pair originally designed for the universal detection of human adenovirus (HAV) serotypes of all subgenera was modified and tested successfully for detecting different bovine, ovine, and porcine adenovirus (BAV, OAV, and PAV, respectively) serotypes. In the examined viruses, parts of the DNA sequence coding for the basal part of the hexon protein were applicable as primers in polymerase chain reaction. Positive amplification was obtained even from sub-group 2 BAVs, which do not cross react with HAVs or subgroup 1 BAVs in Southern hybridization.

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