A human bone marrow cell line (B3HM) inducing leukemia in mouse
Jiang, X.; Ma, Y.; Yang, S.
Zhonghua Zhong Liu Za Zhi 18(2): 105-108
1996
ISSN/ISBN: 0253-3766 PMID: 9206040 Document Number: 466200
Human bone marrow mononuclear cells were cultured on a layer of stromal cells derived from bone marrow of a patient with esophageal cancer. After five-week cultivation, the mononuclear cells could grow independently of stromal cells in liquid culture and had been passaged over 50 generations with a doubling time of 48 hours. The cell line thus established is called B3HM. The growth of B3HM appeared anchorage independent in semi-solid culture and produced 423 +- 87.7 colonies per 5 times 10-4 cells. Immunofluorescence analysis showed that the cells were CD19+, CD20+, CD13+, CD15+, with both B cells and granulocyte surface markers. The cells or cell homogenate could induce leukemia when inoculated in nude mice and "615" inbred mice. The induced mouse leukemia was transplantable and L3T4 positive in more than 80% of the leukemic cells. The pathogenesis of the induced mouse leukemia is worthy of further investigation.