The use of lysolecithin for obtaining a chromosome suspension
Shatrova, A.N.; Aksenov, N.D.; Zenin, V.V.; Poletaev, A.I.
Tsitologiia 38(1): 95-101
1996
ISSN/ISBN: 0041-3771 PMID: 8768555 Document Number: 462447
Lysolecithin treatment was used for obtaining isolated metaphase chromosomes from HeLa cells and lymphoblastoid cell lines BOLD and ROM. Chromosomes were stained with Hoechst 33258 and olivomycine. Chromosome quantity in suspension was determined by dual-laser sorter ATC-3000. It is shown that lysolecithin treatment of the cells allows to isolate chromosomes with higher concentration in suspension and less amount of debris in comparison with Triton X-100 treatment. A protocol for chromosome isolation using lysolecithin treatment is suggested.