Quantification of Endospore Concentrations of Pasteuria penetrans in Tomato Root Material
Chen, Z.X.; Dickson, D.W.; Hewlett, T.E.
Journal of Nematology 28(1): 50-55
1996
ISSN/ISBN: 0022-300X PMID: 19277345 Document Number: 461597
Six methods for quantification of the endospore concentrations of Pasteuria penetrans from tomato roots are described. Mortar disruption and machine disruption methods gave the highest estimations (endospores per gram of root material) of 83.7 and 79.0 million, respectively. These methods were significantly superior to incubation bioassay (47.7 million), enzymatic disruption (32.1 million), and enzymatic disruption + flotation (25.8 million) methods. A centrifugation bioassay method gave the lowest estimation of 12.7 million.