Sterile Culture of Rotylenchulus reniformis on Tomato Root with Gellan Gum as a Supporting Medium
Eyre, M.J.; Caswell, E.P.
Journal of Nematology 23(2): 229-231
1991
ISSN/ISBN: 0022-300X PMID: 19283117 Document Number: 368459
Rotylenchulus reniformis was repeatedly propagated in sterile excised tomato roots growing on modified White's medium with gellan gum as the support. Gellan gum provided an optically clear support medium that could be liquified by adding 5 mM disodium ethylenediaminetetraacetate (EDTA) to facilitate nematode extraction. Liquefaction of the gellan-gum medium by EDTA allowed efficient recovery of eggs and vermiform stages of R. reniformis. Extraction efficiency was quantified with Radopholus similis as a test organism. The efficiency of extracting R. similis from the gellan gum did not vary with the concentrations of EDTA tested.