Surface analysis of IgM-expressing bovine lymphocytes in BLV-infected cattle by immuno-scanning electron microscopy
Levkut, M.; Ponti, W.; Levkutova, M.; Turin, L.; Poli, G.
Folia Histochemica et Cytobiologica 34(1): 31-34
1996
ISSN/ISBN: 0239-8508 PMID: 8773485 Document Number: 459977
Surface of IgM-expressing lymphocytes infected by bovine leukemia virus and with/without persistent lymphocytosis (BLV+PL-, BLV+PL+) in cattle is heterogenous. Three distinct morphological structures of B lymphocytes were found in these groups by immuno-scanning electron microscopy (ISEM). B lymphocytes with generally elongated and pleomorphic microvilli and sometimes short ruffles were observed in infected, and as well as in non-infected animals. A second morphological type endowed with a relatively small number of elongated villi-like veils, and a third morphological type characterized by a relatively small number of short microvilli or blunt stub-like microvilli covering the smooth surface were seen, mainly in BLV+PL+ and seldom in BLV+PL-. Cells of the second type were more intensively labeled, than lymphocytes of the third type.