Improved single-step PCR assay for sex identification post-allogeneic sex-mismatched BMT
Pugatsch, T.; Oppenheim, A.; Slavin, S.
Bone Marrow Transplantation 17(2): 273-275
1996
ISSN/ISBN: 0268-3369 PMID: 8640178 Document Number: 459881
A method is presented which allows sex-identification simultaneously of both male and female cells in cell mixtures. The sensitivity of this PCR-based assay is one male cell in 10(6) female cells. The test can be used routinely to analyze bone marrow and peripheral blood lymphocytes post-allogeneic sex-mismatched bone marrow transplantation to detect early engraftment or rejection, as well as host cell regeneration.
Document emailed within 1 workday
Related Documents
Hou, J.; Cheng, J.; Wang, Z. 1998: Mismatched PCR combined with restriction fragment length polymorphism assay for identification of hot spot for mutation in core promoter sequence of hepatitis B virus in Chinese isolates Zhonghua Yi Xue Za Zhi 78(2): 107-110Tzeng, C.H.; Chen, P.M.; Chiu, C.F.; Chiou, T.J.; Hsien, R.K.; Chuang, M.W.; Chen, K.Y.; Yung, C.H.; Wang, S.Y.; Wang, S.R. 1987: HLA-mismatched allogeneic bone marrow transplantation Zhonghua Yi Xue Za Zhi 39(3): 187-196
Klumpp, T.R.; Herman, J.H.; Mangan, K.F.; Macdonald, J.S. 1993: Graft failure following neutrophil-specific alloantigen mismatched allogeneic BMT Bone Marrow Transplantation 11(3): 243-245
Djuzenova, C.S.; Schindler, D.; Stopper, H.; Hoehn, H.; Flentje, M.; Oppitz, U. 1999: Identification of ataxia telangiectasia heterozygotes, a cancer-prone population, using the single-cell gel electrophoresis (Comet) assay Laboratory Investigation; A Journal of Technical Methods and Pathology 79(6): 699-705
Dunn, J.; O'Connor, T.G.; Levy, I. 2002: Out of the picture: a study of family drawings by children from step-, single-parent, and non-step families Journal of Clinical Child and Adolescent Psychology: the Official Journal for the Society of Clinical Child and Adolescent Psychology American Psychological Association Division 53 31(4): 505-512
Ekins, R. 1992: One-step, labeled-antibody assay for measuring free thyroxine. 1. Assay development and validation Clinical Chemistry 38(11): 2355-2358
Wollin, K-M.; Gorlitz, B-D. 2004: Comparison of genotoxicity of textile dyestuffs in Salmonella mutagenicity assay, in vitro micronucleus assay, and single cell gel/comet assay Journal of Environmental Pathology Toxicology and Oncology: Official Organ of the International Society for Environmental Toxicology and Cancer 23(4): 267-278
Vénuat, A.M.; Marinakis, T.; Bourhis, J.H.; Bayle, C.; Pico, J.L. 1994: Fluorescence in situ hybridization with X and y DNA specific probes for chimerism detection in pulmonary alveolar macrophages after human sex-mismatched allogeneic bone marrow transplantation Bone Marrow Transplantation 14(1): 177-179
Dow, L.W.; Bhakta, M.; Wilimas, J. 1982: Clonogenic assay for Wilms' tumor: improved technique for obtaining single-cell suspensions and evidence for tumor cell specificity Cancer Research 42(12): 5262-5264
Mogi, M.; Motohashi, M.; Hirota, T.; Ohshima, K.; Takahashi, N.; Kinoshita, Y.; Tanigawa, M.; Yoshinobu, T.; Koya, Y.; Masutani, M. 1990: Basic study on the chemosensitivity test by miniaturized improved nucleic acid precursor incorporation assay (MINi assay) Gan to Kagaku Ryoho. Cancer and ChemoTherapy 17(1): 89-95
Godder, K.; Pati, A.; Abhyankar, S.; Gee, A.P.; Parrish, R.; Lee, C.; Henslee-Downey, P.J. 1996: Partially mismatched related donor transplants as salvage therapy for patients with refractory leukemia who relapse post-BMT Bone Marrow Transplantation 17(1): 49-53
Amaral, M.; Rippe, M.P.; Bergoli, C.D.; Monaco, C.; Valandro, L.F. 2011: Multi-step adhesive cementation versus one-step adhesive cementation: push-out bond strength between fiber post and root dentin before and after mechanical cycling General Dentistry 59(5): E185-E191
Cheung, A.N.; Chan, A.C.; Chung, L.P.; Chan, T.M.; Cheng, I.K.; Chan, K.W. 1998: Post-transplantation lymphoproliferative disorder of donor origin in a sex-mismatched renal allograft as proven by chromosome in situ hybridization Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 11(1): 99-102
Johnson, R.B.; Nakamura, R.M. 1978: Simplified Scatchard plot assay for progesterone receptor in breast cancer: comparison with single-point and multipoint assay Clinical Chemistry 24(7): 1170-1176
Fukuda, I.; Kamide, H.; Yoshida, T.; Yoritsune, G.; Kiso, T. 1976: Comparative study of Master's double 2 step test and "augmented" Master's 2 step test, with special reference to Cooper's submaximum single-level load treadmill test Nihon Rinsho. Japanese Journal of Clinical Medicine 34(6): 1266-1273
Smith, M.A.; Jaramillo, Aés.; SivaSai, K.S.R.; Naziruddin, B.; Kaleem, Z.; Patterson, G.Alexander.; Mohanakumar, T. 2002: Indirect recognition and antibody production against a single mismatched HLA-A2-transgenic molecule precede the development of obliterative airway disease in murine heterotopic tracheal allografts Transplantation 73(2): 186-193
Andreas Pfützner; Mirjam Löbig; Fortunato, A.; Forst, T. 2003: Evaluation of a New Fully Automated One-step C-Peptide Chemiluminescence Assay (LIAISON® C-Peptid) Clinical Laboratory 49(5-6): 227-232
Gaither, T.A.; Alling, D.W.; Frank, M.M. 1974: A new one-step method for the functional assay of the fourth component (C4) of human and guinea pig complement Journal of Immunology 113(2): 574-583
Watanaveeradej, V.; Sirirattanaphoomee, S.; Chantratita, W.; Nitayaphan, S.; Viputtikul, K.; Kerdpanich, A.; Samakoses, R.; Simasathien, S. 2005: Quantification of HIV-1 RNA load by one-tube-one-step RT PCR and real time PCR assay with TaqMan probe Journal of the Medical Association of Thailand 88(Suppl 3): S206-S213
Chitapanarux, I.; Kamnerdsupaphon, P.; Tharavichitkul, E.; Sumitsawan, Y.; Sittitrai, P.; Pattarasakulchai, T.; Lorvidhaya, V.; Sukthomya, V.; Pukanhaphan, N.; Traisatit, P. 2008: Effect of oral pilocarpine on post-irradiation xerostomia in head and neck cancer patients: a single-center, single-blind clinical trial Journal of the Medical Association of Thailand 91(9): 1410-1415