Immunohistochemical localization of acidic and basic fibroblast growth factor through corneal neovascularization in vivo and in vitro
Hayashi, N.; Nakayasu, K.; Okisaka, S.
Nippon Ganka Gakkai Zasshi 100(8): 587-591
1996
ISSN/ISBN: 0029-0203 PMID: 8810233 Document Number: 459416
Neovascularization on the center of rabbit cornea was induced by 5N cntdot NaOH alkali burns. We studied the change in localization of acidic fibroblast growth factor (a-FGF) and basic fibroblast growth factor (b-FGF) through corneal neovascularization with immunohistochemistry, using eyes which we enucleated on the 3rd, 7th, and 14th day. Moreover, by co-cultivation of rabbit corneal stromal cells and adrenal cortical vascular endothelium of bovine, a capillary-like code was induced, in which we also studied the localization of a-FGF and b-FGF on the 3rd, 7th, and 14th day. On the 14th day after the alkali burn we recognized intrastromal neovascularization and positive staining of a-FGF and b-FGF around it. Strong staining of a-FGF was observed in goblet cells through the experimental period. In control eyes we recognized positive reaction of a-FGF in corneal and conjunctival epithelium. In co-cultured cells, we recognized positive staining of both a-FGF and b-FGF around the capillary-like code which was induced among the corneal stromal cells.