Distribution of motifs of functional sites in the MDG2 DNA sequence
Ratner, V.A.; Amikishiev, V.G.
Genetika 32(7): 896-901
1996
ISSN/ISBN: 0016-6758 PMID: 8974910 Document Number: 458839
Computer context analysis of the MDG2 (Dm412) DNA sequence was performed, and 530 motifs similar to functional sites from the database were identified. These motifs were found to be nonrandomly distributed along the DNA sequence. Clustering of motifs was revealed in both LTR sequences, in the regions upstream of sORF2, ORF1, and ORF2, and in the spacers between the main polypeptide domains corresponding to ORF2. The total number of clusters found is highly significant due to the presence of a group of the largest "peaks." Internal regions of some ORF contained a decreased number of site motifs (sORF1, ORF1, and ORF2.) In other words, clustering of site motifs was strongly correlated with positions of the regulatory zones in LTR and in the vicinity of the start sites of ORF and their domains. Thus, MGE of Drosophila and other organisms are considered as mobile batteries of functional sites with the increased content of half-finished motifs easily transformed into functional sites. We suggest that these sequences are involved in the adaptive rearrangements of regulatory relationships within molecular genetic regulatory systems.