The nuclease activity of the cell nuclei in 2 lines of murine myeloma sp2/0 differing in their resistance to cytostatics in adriamycin-induced apoptosis
Meliksetian, M.B.; Chirkova, I.V.; Aleksanian, I.T.
Tsitologiia 38(6): 611-615
1996
ISSN/ISBN: 0041-3771 PMID: 9027004 Document Number: 457185
The endonuclease activity of two drug-sensitive and drug-resistant mouse myeloma cell lines during cytotoxic drug-induced apoptosis was studied. It was shown that internucleosomal fragmentation of DNA in drug-sensitive line sp2/0, undergoing apoptosis in the presence of adriamycin and colchicine, was not dependent on intracellular calcium content and was associated with activation of both Ca(2+)-Mg(2+)-dependent and acidic cation-independent endonucleases. In contrast, in multidrug resistant spEBR-5 cells, treated with the same drugs, only Ca(2+)-Mg(2+)-dependent endonuclease activity was detected. These data suggest that the differences in the pattern of endonuclease activity revealed in these cells are linked to drug-resistant phenotype and do not depend on the apoptosis-inducing agent used.