The analysis of grandaksin in cadaveric material
Bodrina, D.E.; Galkina, V.S.; Eremin, S.K.; Salomatin, E.M.
Sudebno-Meditsinskaia Ekspertiza 39(4): 45-47
1996
ISSN/ISBN: 0039-4521 PMID: 9026971 Document Number: 456468
Grandaxine was measured in expert cadaveric material (gastric wall, small intestine, and kidney) by thin layer and liquid chromatography and UV spectroscopy. Chromatographic measurement in a thin layer of the adsorbent was carried out on L 5/40 M plates in the following solvent systems: tholuene-acetone-25% ammonium hydroxide (50:50:1); ethyl acetate-methanol-25% ammonium hydroxide (17:2:1); chloroform-methanol (9:1); and chloroform-acetone (9:1). Analysis by high-pressure liquid chromatography was carried out by means of a Milichrome-4 chromatographer with a UV detector on the column packed with Separone C18 (5 mcm) using a mobile phase acetonitrile-0.05 M disubstituted ammonium phosphate (55:45).