Correlation between expression of mdr-1 gene and oncogenes in human promyelocytic leukemic HL60 cell line and sublines
Zhou, W.D.; Zhang, H.Q.; Fang, M.; Xue, S.B.
Zhongguo Yao Li Xue Bao 17(5): 471-473
1996
ISSN/ISBN: 0253-9756 PMID: 9863178 Document Number: 456200
AIM: To study the relationship between expression of oncogenes and multiple drug resistant (MDR) phenotype. METHODS: The drug resistant level of HL60 cell line and its sublines were determined with flow cytometry. RNA Dot blot hybridization was used to identify the expression of oncogenes and mdr-1 gene. RESULTS: The expression of mdr-1 gene was in the opposite relation with c-myc expression, but in the positive relation with c-H-ras gene expression in the multiple drug resistant cell lines. In nonMDR cell line HL60/RA, the expression levels of mdr-1, c-myc, and c-H-ras were the same as HL60 parental cells. CONCLUSION: Multiple drug resistance is related to not only mdr-1 expression, but also some oncogenes expression level.