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CD45 gating of acute leukemia

Nishikawa, K.; Miyasaki, T.; Tsukaguchi, N.; Noma, Y.; Nakagawa, K.; Narita, N.

Rinsho Byori. Japanese Journal of Clinical Pathology 44(6): 548-553

1996

ISSN/ISBN: 0047-1860
PMID: 8752733
Document Number: 455567
Flow cytometry is a powerful tool for immunophenotyping of acute leukemia. Gating of leukemic cells is currently performed on the two dimensional display of forward scatter (FSC) and side scatter (SSC). However, this gating method can not discriminate leukemic cells from contaminated normal cells. Therefore, we used a CD45 monoclonal antibody to detect leukemic cells (CD45dlm cells) in combination with the SSC parameter. This CD45-SSC gating method was very useful for immunotyping of AML and ALL, especially leukemia with a low percentage of blasts. We recommend this new gating method for more accurate immunophenotyping of acute leukemia.

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