1H NMR investigation of the interaction of berberine and sanguinarine with DNA
Saran, A.; Srivastava, S.; Coutinho, E.; Maiti, M.
Indian Journal of Biochemistry and Biophysics 32(2): 74-77
1995
ISSN/ISBN: 0301-1208 PMID: 7642203 Document Number: 454697
Interaction of the alkaloids, berberine and sanguinarine with calf thymus DNA has been studied by 1H NMR. All proton resonances of the two compounds have been assigned using 2D-COSY, NOESY and ROESY spectra. Berberine has been found to partially intercalate into DNA, while sanguinarine shows normal intercalation and also binds more firmly to DNA. The NMR experiments indicate that sanguinarine is more potent than berberine in its activity.