Methotrexate potentiates anti-herpes simplex virus type 1 activity of E-5- (2-bromovinyl) -2'-deoxyuridine
Pancheva, S.N.
Acta Virologica 39(2): 117-119
1995
ISSN/ISBN: 0001-723X PMID: 7676937 Document Number: 453870
The pharmacologic of induction of low level of deoxyribonucleoside triphosphates (dNTP) is a novel approach to combined inhibition of virus replication. In accordance with this concept the alteration of antiherpes activity of E-5-(2-bromovinyl-2'-deoxyuridine (BVDU) in combination with methotrexate (MTX), an inhibitor which lowers the intracellular pool of thymidine triphosphate (dTTP) was studied. MTX applied alone at non-toxic concentrations had a moderate effect on herpes simplex virus type 1 (HSV-1) replication in human embryonic skin-muscle fibroblasts. The combination of BVDU and MTX acts synergistically, as measured by virus yield assay in the above mentioned system. The potentiating effect of MTX on the anti-HSV-1 activity of BVDU was reversed by thymidine (dThd).