Top Abstract Purchase Related
Home
>
Node
>
Subnode

Automated DNA purification and amplification from blood-stained cards using a robotic workstation

Belgrader, P.; Del Rio, S.A.; Turner, K.A.; Marino, M.A.; Weaver, K.R.; Williams, P.E.

Biotechniques 19(3): 426-432

1995

ISSN/ISBN: 0736-6205
PMID: 7495556
Document Number: 453168
A new method for the purification of DNA on blood-stained cards was developed. This method was implemented into a high-throughput automated system using a Biomek 1000 robotic workstation. In addition, the processes of DNA purification and amplification were coupled into a completely automated and uninterrupted prototype system, and the resultant PCR products generated by this system were subjected to automated desalting for capillary electrophoresis analysis.

Document emailed within 1 workday
Secure & encrypted payments

Caillat-Zucman, S.; Garchon, H.J.; Costantino, F.; Cot, S.; Bach, J.F. 1993: Automation of large-scale HLA oligotyping using a robotic workstation Biotechniques 15(3): 526-528; 530-531
Hochmeister, M.N.; Budowle, B.; Borer, U.V.; Dirnhofer, R. 1995: A method for the purification and recovery of genomic DNA from an HLA DQA1 amplification product and its subsequent amplification and typing with the AmpliType PM PCR Amplification and Typing Kit Journal of Forensic Sciences 40(4): 649
Kostiushov, V.V.; Draznin, G.M.; Ruban, I.A.; Tymchishin, O.L.; Kostiushova, L.A.; Kryshtaleva, A.A. 1991: Use of an automated workstation for clinical and immunological studies and immunodiagnosis in AIDS Voenno-Meditsinskii Zhurnal 10: 15-17; 78
Lockett, S.J.; Jacobson, K.; O'Rand, M.; Kaufman, D.G.; Corcoran, M.; Simonsen, M.G.; Taylor, H.; Herman, B. 1991: Automated image-based cytometry with fluorescence-stained specimens Biotechniques 10(4): 514-519
Buffone, G.J. 1992: Improved amplification of cytomegalovirus DNA from urine after purification of DNA with glass beads Clinical Chemistry 38(11): 2360
Sylvers, L.A.; Beresten, S. 1993: A rapid automated method for simultaneous elution and purification of RNA from polyacrylamide gel Biotechniques 14(3): 378-380
Andrus, A.; Wright, P.; Wang, J.; Mullah, B.; Baier, J.; Mason, J.; Kaufman, G. 1995: Synthesis and purification in a single column on a high-throughput automated oligonucleotide production system Nucleic Acids Symposium Series 34: 183-184
Schmitz, R.; Willeke, F.; Barr, J.; Scheidt, M.; Saelzer, H.; Darwich, I.; Zani, S.; Stephan, D. 2019: Robotic Inguinal Hernia Repair (TAPP) first Experience with the new Senhance Robotic System Surgical Technology International 34: 243-249
Collins, J.N.; Britt, R.C.; Britt, L.D. 2011: Concomitant robotic repair of inguinal hernia with robotic prostatectomy American Surgeon 77(2): 238-239
Ramanujan, R.; Anhalt, M.; Blair, P.; Burdick, B. 1993: Stabilization of nucleic acids in whole blood: an alternative to Guthrie cards Biotechniques 15(5): 825-828
Sciotto, C.G.; Lauer, B.A.; White, W.L.; Istre, G.R. 1983: Detection of Borrelia in acridine orange-stained blood smears by fluorescence microscopy Archives of Pathology and Laboratory Medicine 107(7): 384-386
Bottini, P.B.; Carr, A.A.; Rhoades, R.B.; Prisant, L.M. 1992: Variability of indirect methods used to determine blood pressure. Office vs mean 24-hour automated blood pressures Archives of Internal Medicine 152(1): 139-144
Menichella, G.; Serafini, R.; Ciarli, M.; Paladini, U.; Pierelli, L.; Bunkens, H.; Leone, G. 2001: A new blood donation strategy: automated blood collection (ABC) International Journal of Artificial Organs 24(3): 173-177
Tanwani, S.K.; Pathak, P.N. 1971: Studies on A.B.R. testing. I. Efficacy of tetrazolium stained antigen over haematoxylene stained antigen. II. Comparative use of different methods of milk testing for brucellosis Indian Veterinary Journal 48(3): 229-233
Congpuong, K.; Bualombai, P.; Jitchamroen, S.; Konchom, S. 2001: Comparison of the OptiMAL rapid test with routine microscopic examination of Giemsa-Stained Thick Blood Film for diagnosis of malaria Journal of the Medical Association of Thailand 84(3): 357-363
Cheng, J.M.; Hiemstra, J.L.; Schneider, S.S.; Kaufman, B.A.; Naumova, A.; Cheung, N.K.; Cohn, S.L.; Diller, L.; Sapienza, C.; Brodeur, G.M. 1994: Preferential amplification of the paternal allele in neuroblastomas with N-myc amplification Progress in Clinical and Biological Research 385: 43-49
Sasanakul, W.; Chuansumrit, A.; Rurgkhum, S.; Udomsubpayakul, U.; Hathirat, P. 1999: DNA extraction and amplification of 10-day, room-temperature blood samples Journal of the Medical Association of Thailand 82(Suppl 1): S186-S189
Volin, P. 1992: Determination of urinary normetanephrine, metanephrine and 3-methoxytyramine by high-performance liquid chromatography with electrochemical detection: comparison between automated column-switching and manual dual-column sample purification methods Journal of Chromatography 578(2): 165-174
Golovatskiĭ, A.S. 1979: Dynamic characteristics of the fluorescence of human peripheral blood neutrophilic polymorphonuclear leukocytes and lymphocytes intravitally fluorochrome-stained with acridine orange Tsitologiia 21(4): 480-483
Goto, K.; Yasuno, M.; Ogura, M.; Kobayashi, Y.; Tsukada, T.; Tango, T. 1993: Age and sex dependent distribution pattern and normal range of blood cell parameters obtained by automated blood cell analyzer Rinsho Byori. Japanese Journal of Clinical Pathology 41(10): 1146-1152