PTH-mediated osteoblast retraction: possible participation of the calpain pathway
Murray, E.J.; Tram, K.K.; Spencer, M.J.; Tidball, J.G.; Murray, S.S.; Lee, D.B.
Mineral and Electrolyte Metabolism 21(1-3): 184-188
1995
ISSN/ISBN: 0378-0392 PMID: 7565446 Document Number: 451832
Parathyroid hormone (PTH)-induced osteoblast retraction may play a pivotal role a role in bone resorption by providing osteoclasts direct access to mineralized bone surface. We have been working on the hypothesis that the calpains participate in this retractile response through a calcium-dependent process. We have first demonstrated the presence of calpain activities in MC3T3-E1 osteoblastic cells and that these activities can be stimulated by PTH. Second, we have demonstrated that the PTH-induced osteoblast retraction is dramatically attenuated by two different cysteine protease inhibitors. Finally, initial immunofluorescent cytochemical studies suggest that this PTH-induced osteoblastic retraction is mediated through a calpain-dependent, proteolytic modification of the cytoskeletal organization.