HIV-1 soluble antigens induced CD8+ cytotoxic T-cell responses in an immunized individual
Achour, A.; Moukrim, Z.; Picard, O.; Bizzini, B.; Burny, A.; Zagury, D.
Cellular and Molecular Biology 41(3): 395-400
1995
ISSN/ISBN: 0145-5680 PMID: 7580833 Document Number: 449879
In an attempt to determine whether immunization of healthy HIV- I seronegative individuals with a soluble gp160 candidate vaccine could induce an anti-HIV specific immune response, volunteers were immunized by two injections of a water-in-oil emulsion containing a mixture of gp160 antigen together with selected peptides. Following immunization, lymphocytes were collected and stimulated in vitro with autologous HIV-1-infected cells. The results showed that immunization with soluble HIV-1 envelope was able to generate CD3+ CD8+ CTLs directed to gp 160 antigen. The CTL response was restricted to class I molecule HLA-A2. The CTL response was comparable to that elicited by immunization with HIV-1-envelope recombinant vaccinia virus.