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Detection of methylotrophs in natural samples by amplifying a fragment of the moxF gene

Beliaev, A.S.; Chernykh, N.A.; Gal'chenko, V.F.; Ivanov, M.V.

Mikrobiologiia 64(6): 788-791

1995

ISSN/ISBN: 0026-3656
PMID: 8643037
Document Number: 449807
A method for detection of methylotrophic microorganisms with the help of the polymerase chain reaction was developed. Two primers were synthesized, designed on the basis of comparing conservative sequences of the genes encoding the large subunit of methanol dehydrogenase. The developed system was tested with six strains of methylotrophic bacteria and with natural samples collected in the Bunger Hills oasis, eastern Antarctica. The results suggested a possibility of application of the developed method for qualitative detection of methylotrophs in natural samples.

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