Kinds and characteristics of solid-phase enzyme immunoassay
Sugiura, M.; Mizuoka, K.
Nihon Rinsho. Japanese Journal of Clinical Medicine 53(9): 2188-2191
1995
ISSN/ISBN: 0047-1852 PMID: 7474379 Document Number: 449410
Technology with solid-phase enzyme immunoassay was markedly progressed. Kinds of solid-phase are as follows; (1) tube, (2) beads, (3) latex particle, (4) microtiter plate, (5) ferrite particle, (6) membrane. Recently, application of avidin/biotin system to the tube method was accomplished. As one of the B/F separation with beads method, spin washing method was also developed. Automated open system with the beads method was also developed. Microtiter plate using sponges as the solid-phase was routinely used for allergy tests (CAP-RAST). Multi-Access Open System (MAOS), applicable for various kinds of ELISA kit, will appear in Japan near future. Latex particle with ferrite plating and ferrite particle with glass coating were also used as the solid-phase of EIA. HCV-RIBA II and III systems using nitro-cellulose membrane as solid-phase were also described.