Chemotherapy and recombinant human granulocyte colony-stimulating factor primed donor leukocyte infusion for treatment of relapse after allogeneic bone marrow transplantation
Sica, S.; Di Mario, A.; Salutari, P.; Rutella, S.; Chiusolo, P.; Rumi, C.; Menichella, G.; D'Onofrio, G.; Leone, G.
Bone Marrow Transplantation 16(3): 483-485
1995
ISSN/ISBN: 0268-3369 PMID: 8535325 Document Number: 448319
Two patients affected by acute leukemia relapsed 10 and 12 months respectively after allogeneic bone marrow transplantation. They were treated with aggressive chemotherapy and then infused with HLA-identical donor leukocytes (DLI) collected after recombinant human granulocyte colony-stimulating factor (rhGCSF) administration. A total of 5.6 and 6.3 times 10-6/kg CD34+ cells, 2.7 and 3.0 times 10-4/kg CFU-GM, 4.7 and 4.4 times 10-8/kg MNC, 4.6 and 3.9 times 10-9/kg PMN respectively were infused. Both patients achieved complete remission (CR) and complete chimerism was re-established. One patient developed grade IV acute graft-versus-host disease of the liver requiring immunosuppression and he died in CR from disseminated aspergillosis, 7 months after chemotherapy; one patient is alive in relapse 12 months after treatment.