Organ regeneration and somatic embryogenesis from young stems of Fritillaria sinica
Zhang, H.; Chen, Z.; Yang, L.
Hua Xi Yi Ke Da Xue Xue Bao 26(1): 33-37
1995
ISSN/ISBN: 0257-7712 PMID: 7657334 Document Number: 447736
Regenerated bulbs and embryogenetic calli were inducted from young stems of Fritillaria sinica cultured on Murashige and Skoog (MS) medium supplemented with alpha-naphthale-neacetic acid (NAA), 2,4-dichlophenexy-acetin acid (2,4-D), 6-benzylaminopurine (6BA) and kenetin (Kt) respectively. The callus cultures used for the organ regeneration and somatic embryogenesis were subcultured at intervals of 30 - 40 days on MS medium + NAA 1 mg/L + 6BA 0.5 mg/L. In general, the bulbs can be obtained from cultured Fritillaria sinica through three ways: (1) induction from explants; (2) production of adventitious buds or (3) somatic embryogenesis.