Comparative immunochemical analysis of a complete somatic extract, somatic fractionated and excretory-secretory proteins and antigens of T. spiralis larvae
Odoevskaia, I.M.; Kurnosova, O.P.
Meditsinskaia Parazitologiia i Parazitarnye Bolezni 2007(2): 24-29
2007
ISSN/ISBN: 0025-8326 PMID: 17657951 Document Number: 614874
The paper deals with the analysis of the protein composition of invasion Trichinella larvae, the immunochemical-characteristics of Trichinella antigens of varying molecular mass, the development of the optimum procedures for having the preparative quantities of immunogenic, the so-called major Trichinella antigens that'are the major component for the production of diagnostic and prophylactic agents. Twenty nine protein bands were detected in the composite of a complete somatic Trichinella extract. It was ascertained that the most immunogenic peptides with molecular masses of 30, 43, 52-57, and 63-69 kDa were present in all Trichinella protein-products while peptides with molecular masses of 43, 50-55 kDa are major antigens both in the fractionaied somatic and excretory-secretory proteins in all incubation periods.