Distribution of gamma delta T cells in the pig foetus
Trebichavský, I.; Sinkora, J.; Reháková, Z.; Splíchal, I.; Whyte, A.; Binns, R.; Pospísil, R.; Tucková, L.
Folia Biologica 41(5): 227-237
1995
ISSN/ISBN: 0015-5500 PMID: 8714772 Document Number: 445135
The distribution of gamma delta T cells during different developmental stages of pig foetuses was studied using monoclonal antibodies directed against a ruminant WC1 antigen, a T-cell receptor (TCR) subset epitope, and pig Null T-cell antigen. Binding was revealed by the avidin/biotin/peroxidase technique on cryostat sections and flow cytometry analysis. The extrathymic origin of gamma delta T cells was confirmed: gamma delta T cells appeared in the liver, spleen and peripheral blood sooner than in the thymus. In the course of the second half of gestation, these T cells were found in the dermis and intestinal mucosal compartments which represented the predominant loci of gamma delta T cells after birth.