Autologous mixed leucocyte reaction and the polyclonal activation of bovine gamma/delta T cells
Hanby-Flarida, M.D.; Okragly, A.J.; Baldwin, C.L.
Research in veterinary science 61(1): 65-71
1996
ISSN/ISBN: 0034-5288 PMID: 8819197 Document Number: 462267
Bovine gamma/delta T cells proliferate in response to stimulation with gamma-irradiated autologous monocytes in the autologous mixed leukocyte reaction (AMLR). Flow cytometric analyses indicated that the proliferating cells included three major subpopulations of bovine gamma/delta T cells, distinguished by the differential expression of the gamma/delta T cell receptor epitopes N6 and N7. Interleukin-2 and acid-labile interferon were produced in AMLR cultures but the cultured cells did not lyse any of a large variety of target cells, including monocytes, allogeneic lymphoblasts, transformed bovine B cells (BL3), bovine fibroblast and the natural killer cell targets D17 and K562, even in the presence of lectins or after co-stimulation in the AMLR with antibodies to WC1, the gamma/delta T cell lineage-specific cell-surface differentiation antigen. Ex vivo gamma/delta T cells did not display lymphokine-activated killing whereas populations of peripheral blood mononuclear cells containing alpha/beta T cells did.