Polypeptides from murine peritoneal macrophages recognizing glycosaminylmuramoyl dipeptide
Golovina, T.N.; Sumaroka, M.V.; Samokhvalova, L.V.; Shebzukhov, I.V.; Makarov, E.A.; Nesmeianov, V.A.
Bioorganicheskaia Khimiia 21(4): 268-274
1995
ISSN/ISBN: 0132-3423 PMID: 7786317 Document Number: 444909
By means of radioligand analysis, murine peritoneal macrophages were shown to express several hundreds cell surface high-affinity GMDP-binding sites with a binding constant 350 pM. Photoaffinity labeling followed by SDS-PAGE enabled us to identify inside these cells 32-34 and 38 kDa proteins, specifically binding GMDP. Proteins 32-34 kDa were also detected by Western blotting analysis using biotinylated conjugate of polyacrylamide with immobilized GMDP-Lys [(GMDP-Lys)-PAA-(Bi)] in cell lysate of murine peritoneal macrophages.