The effect of inhibitors of glucose utilization on the chemiluminescence and fluorescence of murine peritoneal macrophages
Kliubin, I.V.
Tsitologiia 34(7): 53-62
1992
ISSN/ISBN: 0041-3771 PMID: 1475853 Document Number: 402963
Using the inhibitors of glucose utilization and mitochondrial oxidation we found that the blue autofluorescence (AF) of murine peritoneal macrophages (MP) originates predominantly from the cytosolic NAD(P)H. AF intensity correlates with the changes in glycolytic enzymes activity. The luminol-dependent chemiluminescence (CL) intensity which characterizes the amount of O-2- generating by MP upon the addition of formyl-methionyl-leucyl-phenylalanine (FMLP) is inhibited in the case of maximum activity of glycolytic enzymes. Incubation of MP with non-convertable analog of glucose, such as 2-deoxyglucose increases the CL intensity under the influence of FMLP. A conclusion is drawn that amount of O-2- generating by MP depends on the intracellular concentration of sugars.