Lymphocyte adhesion to human endothelial cells induces tissue factor expression via a juxtacrine pathway
Schmid, E.; Müller, T.H.; Budzinski, R.M.; Pfizenmaier, K.; Binder, K.
Thrombosis and Haemostasis 73(3): 421-428
1995
ISSN/ISBN: 0340-6245 PMID: 7545320 Document Number: 444548
To study the effect of lymphocyte adhesion on the procoagulant activity of endothelial cells, we have stimulated HUVECs with interferon-gamma to upregulate adhesion molecules. Subsequent addition of lymphocytes induced the expression of tissue factor (TF) by HUVECs. Both CD4+ and CD8+ T-cells promoted this TF synthesis via distinct adhesion molecules (CD4+ T-cells: E-selectin and ICAM-1; CD8+ T-cells: MHC-I molecules). In addition, tumor necrosis factor-alpha and -beta (TNF-alpha, TNF-beta) and platelet-activating factor (PAF) were involved in lymphocyte-mediated TF expression on HUVECs. We demonstrate that PAF plays a pivotal role in this process. Adhesion of lymphocytes to endothelial cell surface molecules induced the release of PAF. PAF, in turn, caused the production of TNF-alpha and TNF-beta, both of which are potent stimulators of TF expression.