Thyroid status and potassium currents in rat ventricular myocytes
Shimoni, Y.; Severson, D.L.
American Journal of Physiology 268(2 Pt 2): H576-H583
1995
ISSN/ISBN: 0002-9513 PMID: 7864182 Document Number: 441696
The size and rate dependence of the transient (I-to) and steady-state (I-ss) outward potassium currents were investigated in isolated rat ventricular myocytes obtained from euthyroid, hyperthyroid, and hypothyroid rats, using the whole cell, suction electrode voltage-clamp method. Under hypothyroid conditions I-to was reduced in size, with no significant change in I-ss. In hypothyroid cells, the rate dependence of both currents was greatly enhanced, resulting in a much larger attenuation with increasing stimulation rates. A significant slowing of the recovery kinetics of I-to was observed. These effects of hypothyroidism were reversed by physiological triiodothyronine (T-3) replacement. These current changes were reflected in an altered rate dependence of the action potential configuration in hypothyroid myocytes. Under hyperthyroid conditions no significant changes were observed in the amplitude or time course of recovery of I-to. I-ss amplitude was increased, but no changes were found in its rate dependence. These results are discussed in terms of hormonal, long-term modulation of potassium currents, and in terms of cardiac pathology under conditions of altered thyroid status.