Evaluation of treatment for the prevention of acute gastric lesions in stress, in rats
Laudanno, O.M.; Bedini, O.A.; Cesolari, J.A.; San Miguel, P.
Acta Gastroenterologica Latinoamericana 25(3): 145-152
1995
ISSN/ISBN: 0300-9033 PMID: 8600701 Document Number: 440372
To verify wether a cytoprotective or a antisecretant drug is effective on stress acute gastric. Female Wistar rats (n=7), 200 g, 24 h fasted, water ad lib. were used. Experiment I. Stress (s) by immobilization and immersion in 181/3 C water, 6 h (control). 30 min pretreatment with 2 ml 800 mg/Kg in OG bolus Sucralfate. 800 mg/Kg OG Magaldrate. 800 mg/Kg OG AIMgOH. 800 mg/Kg OH Hydrotalcite. 100 aeg/Kg OG Misoprostol (antisecretant). 125 mg/Kg s.c. Somatostatine (Octeotride). 30 mg/Kg i.p. Ranitidine. 30 mg/Kg i.p. Omeprazole and 30 mg/Kg i.p. Lanzoprazole. The rats were sacrificed by ether overdose, and laparotomy was performed to remove stomach and to assess the percentage gastric lesional area by computerized planimetry and histological examination. Experiment II. With the same scheme above described and previous anesthesia, laparatomy and pylorus ligature, the acid gastric secretion was studied. Results Exp. I. Percentage of gastric lesional area: C: 80.5 +/- 5.1; Sucralfate: 25.6 +/- 4.1 (p < 0.05). Magaldrate: 51.6 +/- 7.1 (NS). AIMgOH 78.6 +/- 7.1. Hydrotalcite; 85.6 +/- 5.1. Misoprostol (cytoprotector) 59.5 +/- 6.1 (NS). Misoprostol (antisecretant) 1 +/- 0.1 (p < 0.001). Somatostatine: 20.3 +/- 5.1 (p < 0.01). Ranitidine: 46.5 +/- 7.8 (NS). Omeprazole: 1 +/- 0.1 and Lanzoprazole: 1 +/- 0.1 (p < 0.001). Histological examination demonstrated gastric cytoprotection in the groups pretreated with Omeprazole and Lanzoprazole. Exp. II. 6 h m/Mol HCl output: C: 0.22 +/- 0.09. Sucralfate: 0.2 +2- 0.07 (NS). Magaldrate: 0.19 +/- 0.08. AIMgOH: 0.18 +/- 0.07. Hydrotalcite: 0.19 +/- +/- 0.10. Misoprostol (cytoprotector): 0.23 +/- 0.07. Misoprostol (anti-secretor): 0.11 +2- 0.05 (p < 0.05). Somatostatin: 0.09 +/- 0.04. Ranitidine: 0.08 +/- 0.05. Omeprazole: 0.02 +/- 0.01 (p < 0.001) and Lanzoprazole: 0.02 +/- 0.01. In the prevention of stress acute gastric lesions Sucralfate might be indicated as a partial cytoprotector and Omeprazole or Lanzoprazole as antisecretant and lesion preventive drugs.