Scanning microscopic evaluation on the development of the cerebral cortex in embryonic mouse subjected to gamma-irradiation
Sun, X.Z.; Inouye, M.; Hayasaka, S.; Takagishi, Y.; Yamamura, H.
Environmental Medicine Annual Report of the Research Institute of Environmental Medicine Nagoya University 39(2): 117-120
1995
ISSN/ISBN: 0287-0517 PMID: 12703528 Document Number: 439273
Morphological events occurring in the developing cerebral hemispheres of mice exposed to a single dose of 60Co gamma-irradiation 1.5 Gy on embryonic day 13 (E13) were evaluated by scanning microscope. Twenty-four hr after the exposure, both cell debris and surviving cells had poured out into the ventricular lumen. Radial glial fibers were more crumpled than in the controls. By day E15, proliferating cells in different stages of the cell cycle appeared in the ventricular zone. The glial fibers formed a network through the brain mantle. By E17 many migrating cells attached to the disorderly glial fibers appeared in the different layers of the thin cerebral mantle. These findings suggest that development of the glial fibers was interrupted as early as 24 hr after the single exposure, meaning that irradiation on the developing brain may disrupt neuronal migration.