The effects of transcorneal administration of prostaglandin E2 on rabbit eyes
Hirata, H.; Hiraki, S.; Kaji, Y.; Takeda, N.; Fukuo, Y.; Tachinami, K.
Nippon Ganka Gakkai Zasshi 98(10): 927-934
1994
ISSN/ISBN: 0029-0203 PMID: 7976827 Document Number: 438623
The pharmacologic effects of prostaglandin E-2 (PGE-2) on the anterior ocular segment were investigated. PGE-2 was administered with a glass cylinder attached to the cornea in concentrations ranging from 0.05 to 500 mu-g/ml. The intraocular inflammatory reaction was assessed according to the changes in the anterior chamber flare, ocular tension, and components of the aqueous humor. We also performed experiments designed to inhibit the reactions induced by PGE-2 using anti-inflammatory agents. The PGE-2 elicited a single peak reaction in the anterior chamber flare, which recovered after several hours, and a transient elevation in the ocular tension in the early stage after PGE-2 administration. Quantitative analysis of these two reactions revealed that both were dependent on the dose of PGE-2 administered. The concentrations of ascorbic acid and glutathione, particularly that of the reduced that of the reduced form of the latter, were transiently reduced it the aqueous humor. The anterior chamber flares were suppressed by agents that inhibit arachidonic acid metabolism. These findings suggest that the metabolism of arachidonic acid becomes newly activated as a result of stimulation by PGE-2 of extrinsic origin.