Expression of transforming growth factor-beta 1-4 in the chick limb bud mesenchymal cells: regulation by 1,25-dihydroxyvitamin D3
Del Rio-Tsonis, K.; Tsonis, P.A.
Biochemical and Biophysical Research Communications 204(2): 975-982
1994
ISSN/ISBN: 0006-291X PMID: 7980568 Document Number: 438380
When mesenchymal cells from the early chick limb bud (stage 23-24) are plated at high cell density they spontaneously undergo chondrogenesis, implicating extensive cell-to-cell interactions. In the past it has been shown that TGF-beta or 1,25 dihydroxyvitamin D-3 influences this process. In the present study we investigated the expression of TGF-beta isoforms in the mesenchymal cells after treatment with 1,25-dihydroxyvitamin D-3. We found that TGF-beta-2 was down-regulated due to the treatment. The size of the transcripts was the expected as described for sternum cartilage-cells but with differential use preference.