Significance of DNA endonucleases in the pathogenesis of ischemic lesion of the brain during massive hemorrhage
Kozhura, V.L.; Nosova, N.V.; Novoderzhkina, I.S.; Solov'eva, Z.V.
Anesteziologiia i Reanimatologiia 5: 31-33
1994
ISSN/ISBN: 0201-7563 PMID: 7893073 Document Number: 435337
Experiments on dogs exposed to hemorrhagic shock demonstrated a direct relationship between the duration of arterial hypertension and activities of Ca(2+)-Mg(2+)-dependent DNA-endonucleases in the cellular nuclei of the cerebral cortex. Appreciable differences in the kinetics of accumulation of acid-soluble products of DNA chromatin in the cellular nuclei of the cerebral cortex were detected: chromatin DNA degradation was the most rapid in the nervous tissue of dogs exposed to 6 h hemorrhagic shock. Verapamil, a Ca blocker, injected to animals 30 min before massive hemorrhage in a dose of 0.1 mg/kg, reliably reduced the activities of Ca(2+)--Mg(2+)-dependent endonucleases of the cellular nuclei of the cerebral cortex by the 4th h of hemorrhagic shock. The detected physicochemical changes in the chromatin DNA of the cerebral cortex cellular nuclei and a progressive increase of activities of Ca(2+)--Mg(2+)-dependent endonucleases during a prolonged hemorrhagic shock are by their nature close to the phenomenon of genomic programmed cellular death.