Mixed-mode fluorescent DNA sequencing
De Bellis, G.; Consani, I.; Caramenti, G.; Pergolizzi, R.; Debernardi, S.; Invernizzi, L.; Luzzana, M.
Biotechniques 16(6): 1112-1115
1994
ISSN/ISBN: 0736-6205 PMID: 8074877 Document Number: 434292
Fluorescence-based, automated DNA sequencers represent one of the major advances in recent molecular biology. Two main technologies have been developed in this field: the single-label/four-lane system and the four-label/one-lane system. The following presents the use of single-label sequencing chemistry, which resembles traditional radioactive DNA sequencing, using the four-label system ABI 373A that expands its flexibility and obtains data that are immediately interpretable without software manipulation. This method has been named mixed-mode fluorescent DNA sequencing. Here we show one of its possible applications in molecular genetic analysis.