Effect of cyproterone acetate on the proteinase activities of adult rat testis and epididymis
Nag, S.; Sarker, D.; Ghosh, J.J.
Biochemical Pharmacology 26(1): 5-6
1977
ISSN/ISBN: 0006-2952 PMID: 12762 DOI: 10.1016/0006-2952(77)90121-6Document Number: 434133
The in vivo and in vitro effects of cyproterone acetate (CA), an antiandrogenic compound, on the proteinase activities in epididymal and testicular spermatozoa in male albino rats was studied. CA was injected intramuscularly at a dose of 50 mg/kg daily for 10, 20, and 30 days. The testis and epididymis were homogenized and submitted for enzyme assay. The in vitro experments involved the incubation of supernatents from centrifuged testis and epididymis for 30 minutes with CA. Proteinases were assayed using acid-denatured hemoglobin as substrate. Acid proteinase activities increased in both testis and epididymis, but the inhibition of neutral and alkaline proteinase activities was greater in epididymis than testis with both long- and short-term treatment with CA. It is suggested that CA inhibits the maturational processes in the epididymis rather than spermatogenesis in the testis.
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