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An in vitro and in vivo study of antitumor effects of rHTNF-alpha on human ovarian cancer

Zhao, E.F.; Zhou, M.Q.; Fu, C.Y.

Zhonghua Zhong Liu Za Zhi 16(4): 273-276

1994

ISSN/ISBN: 0253-3766
PMID: 7805556
Document Number: 432985
We examined the cytotoxic activities of recombinant human tumor necrosis factor (rHTNF-alpha) and five chemotherapeutic agents, CTX, 5-Fu, VCR, DDP, KSM, against two human ovarian cancer cell lines, OVCAR-3 and CAOV-3, using the MT assay. The results showed that cytotoxicities of rHTNF-alpha at 5 times 10-5 times 10-4 u/ml against OVCAR-3 cell line for 24 h exposure were from 14.2 +- 6.8% to 67.2 +- 3.0%, and those against CAOV-3 cell line were from 8.2 +- 4.3% to 60.9 +- 1.3%. The cytotoxic effects of all five chemotherapeutic agents against the two cell lines were much lower than that of rHTNF-alpha. Further, we studied the combined anticancer potential of rHTNF-alpha with chemotherapeutic agents against the two cell lines. Various degrees of synergism in cytotoxicities of DDP or KSM in combination with rHTNF-alpha were observed. The cytotoxic effect of rHTNF-alpha on CAOV-3 cell were also morphologically observed under phase contrast and electron microscope. Based on experiment in vitro, the in vivo anticancer activity of rHTNF-alpha alone or in combination with KSM was examined against human ovarian cancer OVCAR-3 subcutaneously transplanted in nude mice. After 8 weeks of treatment, a statistically significant difference of mean tumor volume was found between the control group and groups that received rHTNF-alpha or rHTNF-alpha plus KSM (P lt 0.01).

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