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DNA probes for identifying the members of the Anopheles punctulatus complex in Papua New Guinea

Beebe, N.W.; Foley, D.H.; Saul, A.; Cooper, L.; Bryan, J.H.; Burkot, T.R.

American Journal of Tropical Medicine and Hygiene 50(2): 229-234

1994

ISSN/ISBN: 0002-9637
PMID: 8116817
Document Number: 432719
Genomic DNA probes were made for five members of the Anopheles punctulatus complex of mosquitoes found in Papua New Guinea. Specific DNA probes were developed for An. punctulatus, An. koliensis, and three sibling species, An. farauti No. 4, An. farauti No. 5, and An. farauti No. 6, by differentially screening total genomic DNA libraries of individual species and sibling species with homologous DNA against heterologous DNA labeled with 32P. Probes ranged from 273 to 630 bp. Identification of species can be made from squash or dot blots using only a segment of the mosquito (i.e., head, thorax, abdomen, or even legs), allowing for concurrent analysis of the remainder of the mosquito for other epidemiologic characteristics.

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